Global metabolomics by chemical isotope labeling LC-MS


Description

This service aims at comprehensively and quantitatively exploring the whole metabolome without any prior information, providing a global view of a sample. It tries to detect, quantify and compare as many metabolites as possible between samples without bias.

To increase metabolome coverage and achieve accurate quantification for all detectable metabolites, we use a Chemical Isotope Labeling (CIL) Metabolomics Platform for metabolome analysis. The whole metabolome is analyzed by combining the analysis of four submetabolomes: amine/phenol, carboxyl, carbonyl and hydroxyl submetabolome. The combined results from four channels are able to cover 85% to 95% of the entire chemical space of the metabolome. However, a user can choose the labeling method(s) to analyze one or more chemical-group-based submetabolomes, depending on budget and desired coverage. Using one submetabolome analysis, we detect and perform accurate relative quantification of up to 2,500 metabolites (not features) in many different types of samples, including urine, blood, plant extract and cell extracts. Using a combination of four submetabolome analysis, we can cover over 7,000 to 10,000 metabolites (sample dependent) per sample.

For the analysis, we will do sample preparation, metabolite extraction, chemical labeling, LC-MS analysis, data analysis, metabolite ID, and statistical analysis (PCA, PLS-DA, Volcano plots). We will provide a summary of our analysis and statistical results. The peak pairs (metabolites) detected, positive or putative IDs, and their relative quantification data will be summarized in an Excel file, which can be used for further analysis such as uploading to MetaboAnalyst for more statistical analysis or pathway analysis.


Price

  • $90 USD for first labeling
  • $70 USD for additional labeling
  • Four labelings: $90+3x$70 = $300 USD per sample
  • The above price applies to sample size of =>24. If the sample number is less than 24, add 20% extra.
  • $75 USD per hour for additional data analysis beyond those included in the report
  • Academic/government: no overhead
  • Industry: add 30% university overhead

Minimum Volume

20-100 ┬ÁL or 1-5 mg lyophilized powder