Mevalonate Pathway and Isoprenoids/Cholesterol Synthesis


Description

Detection and quantitation of isoprenyl phosphate intermediates, isoprenoids (ubiquinones, dolichols, vitamin K2 and squalene, etc.), cholesterol and other sterols by UPLC-MRM/MS and UPLC-FTMS.

Quantitative

Absolute

Platform Available

UPLC-MRM/MS


Price

*Academic/Government: $400 1st sample; $180 each additional sample * *Industry: $800 1st sample; $360 each additional sample

Minimum Volume

  • Tissue: 50 mg
  • Cell: 5x10^6
  • Blood/plasma/serum: 100 µL
  • Urine: N/A
  • Plant: N/A
  • Feces: 50 mg
  • DBS: 30 µL


NameHMDB IDSuper ClassClass
3-Hydroxy-3-methylglutaryl CoA (HMG-CoA)
Dolichols ( 13 to 21 isoprene units)
Isopentenyl pyrophosphate
Lathostenol
Menoquinones (vitamin Ks)
Mevalonic acid 5-phosphate
Ubiquinones ---CoQ6, CoQ8, CoQ9, CoQ10, CoQ11, CoQ12
2,3-oxidosqualeneLipids and lipid-like moleculesPrenol lipids
7-Dehydrocholesterol (provitamin D3)Lipids and lipid-like moleculesSteroids and steroid derivatives
7-DehydrodesmosterolLipids and lipid-like moleculesSteroids and steroid derivatives
Acetoacetyl-CoALipids and lipid-like moleculesFatty Acyls
Acetyl-CoALipids and lipid-like moleculesFatty Acyls
CholesterolHMDB00067Lipids and lipid-like moleculesSteroids and steroid derivatives
DesmosterolHMDB0002719Lipids and lipid-like moleculesSteroids and steroid derivatives
DihydrolanosterolLipids and lipid-like moleculesPrenol lipids
Dimethylallyl pyrophosphateLipids and lipid-like moleculesPrenol lipids
Farnesyl pyrophosphateLipids and lipid-like moleculesPrenol lipids
Geranyl pyrophosphateLipids and lipid-like moleculesPrenol lipids
Geranylgeranyl pyrophosphateLipids and lipid-like moleculesPrenol lipids
LanosterolLipids and lipid-like moleculesPrenol lipids
Mevalonic acidHMDB0059629Lipids and lipid-like moleculesFatty Acyls
SqualeneLipids and lipid-like moleculesPrenol lipids
ZymostenolLipids and lipid-like moleculesSteroids and steroid derivatives
ZymosterolLipids and lipid-like moleculesSteroids and steroid derivatives
Mevalonic acid 5-pyrophosphateOrganic oxygen compoundsOrganic oxoanionic compounds