Services

TMIC offers custom tailored metabolomics services that are many times faster, more quantitative, and far more comprehensive than any other facility in North America and provides a single source destination for fee-for-service metabolic profiling including comprehensive metabolite identification, quantification, and analysis for the medical, agricultural, food processing (including functional food and nutraceutical) as well as the environmental testing markets.

Available Technical Services

TMIC offers the following technical services for the analysis of biofluids, tissues, bioproducts, bioprocesses, or crops by GC-MS, LC-MS, NMR, HPLC and combined approaches:

  • Lipidomics (phospholipids, TAGs, DAGs, MAGs, CEs, and fatty acids)
  • Phytochemical analysis
  • Nutrients (general metabolite analysis)
  • Metabolic footprinting and fingerprinting
  • Food composition analysis
  • Biofuel composition analysis
  • Tissue imaging

TMIC has comprehensive experience working with many types of samples and model organisms. If your organism or sample type does not appear on these lists, please contact us to see if we can still accommodate your needs.

TMIC Metabolomics Services

Service TypeInstrument(s)Volume
Quantitative Direct Flow Injection Mass Spectrometry (DI-MS)QTrap 4000 MS20 - 100 uL
Quantitative NMR Spectroscopy500, 600, 800 MHz200 uL to 1 mL
Quantitative GC-MSGC-MS, GC-TOF100 uL
Targeted, Quantitative LC-MSUPLC-FTMS20-50 uL
Untargeted LC-MS or DI-MSUPLC-FTMS, UPLC-ESI-MS/MS50 uL
Quantitative LipidomicsHPLC-ELSD with FAMES/GC-MS500 uL to 1 mL
Polyphenol AnalysisHPLC200 uL
Anthocyanin/Chlorophyll AnalysisHPLC200 uL
Bile Acid AnalysisHPLC/LC-MS200 uL
Thiol AnalysisHPLC200 uL
Vitamin AnalysisHPLC400 uL
Nucleoside/Nucleotide AnalysisHPLC200 uL
Exotic Lipid AnalysisHPLC200 uL
Biogenic AminesHPLC/DI-MS20-200 uL
Metabolite ImagingMALDI-TOF/TOFN/A
Central Carbon Metabolism AnalysisUPLC-FTMS200 uL
Metal Analysis (Metalomics)ICP-MS200-500 uL

TMIC Assays

GC-MS

GC-MS Sample Analysis

  • Done using Agilent 5695 GC-MS system
  • Minimum sample volume of 30 µL
  • Lower limit of detection ~500 nM
  • 20-70 metabolites identified and quantified depending on sample
  • Capable of analyzing biofluids (urine, blood, CSF, bile, rumen, saliva, etc.), tissue extracts (plant or animal), beverages, foods or food products

GC-TOF-MS Sample Analysis

  • Done using Waters GC-TOF-MS system
  • Minimum sample volume of 30 µL
  • Lower limit of detection ~500 nM
  • 40-100 metabolites identified and quantified depending on sample
  • Capable of analyzing biofluids (urine, blood, CSF, bile, rumen, saliva, etc.), tissue extracts (plant or animal), beverages, foods or food products

NMR

500 MHz NMR Sample Analysis

  • Done using Varian 500 MHz NMR with cold probe
  • Minimum sample volume of 100 µL
  • Lower limit of detection ~5 µM
  • 20-70 metabolites identified and quantified depending on sample using 1D NMR

600 MHz NMR Sample Analysis

  • Done using Varian 700 MHz NMR with cold probe
  • Minimum sample volume of 100 µL
  • Lower limit of detection ~5 µM
  • 25-75 metabolites identified and quantified depending on sample using 1D NMR

HPLC-MS

Melatonin analysis

  • Done using HPLC and extractions
  • Minimum sample volume of 200 µL
  • Lower limit of detection ~10 pg/mL

Specialized compound analysis (phytochemicals, CoA derivatives, metabolite intermediates)

  • Done using HPLC, extractions and selective chemistry
  • Minimum sample volume of 30 µL
  • Lower limit of detection ~100 nM
  • 20-40 metabolites identified and quantified depending on sample

LC-MS

DI-MS of prepared liquid samples

  • ABI 4000 Q Trap ESI-MS/MS
  • Minimum sample volume of 20 µL
  • Lower limit of detection ~5 nM
  • 100-160 metabolites identified and quantified depending on sample

LC-MS Isotope Labeling Sample Analysis

  • Done using HPLC
  • Minimum sample volume of 10 µL
  • Lower limit of detection ~20 nM
  • 40-200 metabolites identified and quantified depending on sample

Lipidomics

  • Done using HPLC-ELSD, FAMES, GC-MS and CLR
  • Minimum sample volume of 200 µL
  • Lower limit of detection ~1 nM
  • 1000-4000 lipids identified and quantified depending on sample

Other Services

Assistance with multivariate statistical data analysis

  • Analytical and statistical interpretive services
  • Tissue imaging

Sample extraction