Equipment


With access to over $26 million in state-of-the-art metabolomics infrastructure, TMIC is able to offer the most comprehensive set of technologies for targeted/quantitative and untargeted metabolomics using NMR, GC-MS, LC-MS, UPLC, HPLC and metabolite imaging. TMIC is able to process 700 samples/week using as little as 20 µL of material. We continuously monitor the status and output quality of all our instruments to guarantee consistent and high quality results. Our scientists and technicians are fully trained in standard operating, maintenance and quality assurance procedures, and have many years experience in handling a wide range of samples and experimental procedures.

See here for the different services we offer and background on the science behind them.





List of Major Equipment in TMIC :

Equipment TypeFunded ByIn Operation as of (mm/yyyy)
Thermo Fusion Orbitrap (UVic)Genome Canada11/2013
Thermo Velos Pro Orbitrap (UVic)Genome Canada / Western Economic Diversification (WD)11/2011
Upgraded 11/2013
Agilent 6490 with MALDI source (UVic)UVic-Genome BC Proteomics Centre01/2013
Agilent 6490 QQQ (UVic)Genome Canada / WD01/2011
Agilent 6490 QQQ (UVic)Genome BC, UVic-Genome BC Proteomics Centre08/2011
AB QTRAP 4000 (UVic)Enabling Technologies01/2007
AB QTRAP 4000 (UVic)UVic-Genome BC Proteomics Centre04/2008
AB QTRAP 2000 (UVic)Western Economic Diversification04/2011
AB 4800 MALDI TOF/TOF (UVic)Genome Canada06/2006
AB Voyager DE-STR MALDI TOF (UVic)Natural Sciences and Engineering Research Council (NSERC)01/2000
Bruker Ultraflex III MALDI TOF/TOF (UVic)WD, UVic-Genome BC Proteomics Centre06/2009
Bruker 12T FT-MS (UVic)Genome Canada, UVic, BC Gov’t01/2007
Waters Synapt HDMS (UVic)WD, UVic-Genome BC Proteomics Centre04/2008
Agilent 1290 HPLC (UVic)Genome Canada, UVic, BC Gov’t02/2013
Agilent 1290 HPLC (UVic)Genome Canada, UVic, BC Gov’t02/2013
Agilent 1290 HPLC (UVic)Genome Canada, UVic, BC Gov’t02/2013
Agilent 1290 HPLC (UVic)Genome Canada, UVic, BC Gov’t02/2013
Waters NanoAcquity UPLC system (UVic)WD, UVic-Genome BC Proteomics Centre06/2008
Waters NanoAcquity UPLC system (UVic)WD, UVic-Genome BC Proteomics Centre06/2008
Waters NanoAcquity UPLC system (UVic)WD, UVic-Genome BC Proteomics Centre06/2008
Agilent Bravo Liquid Handling System (UVic)Genome Canada, UVic, BC Gov’t01/2013
Bruker 800MHz NMR with cryoprobe (UofA)NSERC, CIHR, AHFMR, UofA and CFI06/1999
(upgrade 09/2016)
Bruker 700 MHz NMR with cryoprobe and autosampler (UofA)Western Economic Diversification09/2015
Varian/Agilent 600 MHz NMR with autosampler (UofA)NSERC, Canadian Institutes of Health Research (CIHR), AHFMR, UofA06/2000
Varian/Agilent 500 MHz NMR with cryoprobe (UofA)CFI (Prion Centre)08/2013
Varian/Agilent 500 MHz NMR with cryoprobe (UofA)NSERC, CIHR, AHFMR, UofA06/1999
ABI QTRAP 4000 MS with Turbo ESI (UofA)CFI, AAET06/2008
ABI QTRAP 4000 MS with Turbo ESI (UofA)Alberta Innovates – Health Solutions (AIHS)07/2014
Bruker Ion TRAP MS with Agilent HPLC system (UofA)CFI, AAET06/2007
Bruker maXis II QTOF (UofA)Bruker, Canada Research Chairs02/2013
Bruker maXis II QTOF (UofA)Western Economic Diversification08/2015
Waters Synapt HDMS/QTOF (UofA)Waters, IROA06/2016
Waters HDMS/QTOF (UofA)NSERC08/2015
AB 4800 MALDI TOF/TOF (UofA)CFI, Alberta Advanced Education and Technology (AAET)06/2008
Bruker 9.4 Tesla FT-ICR with HPLC and ESI/MALDI sources (UofA)CFI, Alberta Advanced Education and Technology (AAET)06/2008
Bruker Ion Trap MS with Agilent HPLC system (UofA)CFI, AAET06/2007
Perkin-Elmer NexIon 350XX ICP-MS (UofA)Genome Canada03/2016
Bruker SCION TQ GC456 GC-MS/MS with cryo cooling and backflush option (UofA)NSERC12/2012
Leco GCxGC FID/TOF-MS with liquid N2 cryogenic quad jet modulator (UofA)CFI, UofA, Leco Instruments, Alberta Small Equipment Grants Program (SEGP)07/2009
Agilent 7890A GC-MS with autosampler (UofA)National Research Council - National Institute for Nanotechnology (NRC-NINT)06/2005
Agilent 7890A GC-MS with autosampler (UofA)Genome Canada01/2014
Agilent/HP Series 5890 GC-MS (UofA)AAET, Alberta Livestock and Meat Agency (ALMA)08/1990
Beckman System Gold HPLC with UV detector (UofA)AHFMR, UofA09/1996
Beckman System Gold HPLC with ELSD (UofA)Genome Canada, AAET06/2006
Agilent 1290 HPLC with Fluorescent detector (UofA)AAET, ALMA06/2009
Agilent 1290 HPLC with Fluorescent detector (UofA)External Revenue01/2014
Waters NanoAcquity UPLC system with UV detector (UofA)Genome Canada06/2007
Waters UHPLC system with UV detector (UofA)NSERC03/2011
Agilent 1290 HPLC system (UofA)Genome Canada02/2013
Agilent 1290 HPLC system (UofA)AAET, ALMA06/2009
Biomek 2000 Liquid Handling System (UofA)NSERC/CFI02/2008
Biomek 2000 Liquid Handling System (UofA)Genome Canada05/2015


NMR

In NMR-based metabolomics, spectral profiling or spectral deconvolution is frequently used to identify and quantify metabolites from 1D 1H NMR spectra. TMIC uses Chenomx Spectral Profiling software to identify and quantify metabolites from 1D 1H NMR spectra. TMIC has also developed a fully automated and quantitative NMR spectral profiling system, BAYESIL. Based on extensive testing with defined mixtures and real biological samples BAYESIL consistently performs with sensitivity and specificity greater than 98% for compound identification in mixtures with up to 60 different compounds. It also determines metabolite concentrations (down to 10 µM) within 10% of the known or expert-measured concentrations. In other words, BAYESIL operates at a level that meets or exceeds the performance of the most highly trained human experts. BAYESIL appears to be the first system that supports fully automated and fully quantitative NMR-based metabolomics. Using NMR-based metabolomics, it is possible to identify and quantify up to 209 metabolites in urine, 55 metabolites in blood, 50 metabolites in CSF, and 45 metabolites in cell or tissue extracts in a single analysis. NMR is ideal for studying amino acids, organic acids, amines, sugars and alcohols.

Find out more about NMR here.

TMIC currently houses the following NMR instrumentation:

  • 600 varian nmr rtprobe

    600 MHz Varian NMR with 5mm RT probe (2 instruments)

  • 500 varian nmr coldprobe

    500 MHz Varian NMR with 5mm cold probe

  • 700 MHz Varian NMR with 5mm cold probe

  • 800 varian nmr coldprobe

    800 MHz Varian NMR with 5mm cold probe


GC-MS

TMIC has developed multiple extraction techniques and uses external calibration-based quantitation for GC-MS analyses. AutoGC software is being developed by TMIC which will semi-automatically identify and quantify metabolites. The NIST08 GC-MS and Golm Metabolome Databases are also used for metabolite identification. With our GC-MS methods, identification and quantification of up to 100 metabolites in urine, 40 metabolites in blood, 60 metabolites in cerebrospinal fluid, and 35 metabolites in cell or tissue extracts is possible. GC-MS is ideal for studying amino acids, biogenic amines, primary amines, organic acids and sugars.

We also offer GC-MS-based lipidomics, using reverse phase HPLC-ELSD to separate lipid classes into 7 groups followed by FAMES and GC-MS analysis to identify and quantify the component fatty acid chains. TMIC-developed Combinatorial Lipid Reconstruction software is used to reconstruct lipid molecules and calculate their concentrations. These lipidomics methods are ideal for identifying/quantifying fatty acids, neutral lipids, phospholipids, and sphingolipids.

Additionally, separation, identification and quantification of small volatile and semi-volatile molecules by two-dimensional GC-MS is offered by TMIC. GCxGC is the gold standard in separating and quantifying exceedingly complex samples of volatile organic compounds, which play a key role in food, flavour and aroma metabolomics, as well as plant and animal communication.

Find out more about GC-MS here.

The following GC-MS and GCxGC instruments are available at TMIC:

  • Agilent 5890 II GC-quad MS with sample changer

  • Agilent 5695 gcms

    Agilent 5695 GC-quad MS with sample changer

  • Gcxgc fid tofms

    GCxGC FID/TOFMS equipped with a liquid nitrogen cryogenic quad jet modulator

  • Bruker scion tq gc456 gcms

    GC-MS/MS Bruker SCION TQ GC456 cryo cooling on the PTV, backflush option available


FT-ICR MS

FT-ICR MS offers very high resolution and high mass accuracy, making it an ideal technology for metabolomics studies of complex samples, and identification of unknown compounds. TMIC developed software, MyCompoundID, and TMIC databases are used for identification of previously unknown or predicted metabolites from high resolution MS data.

TMIC's FT-MS instruments include:

  • Bruker 9 4 tesla fticr ms

    Bruker 9.4 Tesla FT-ICR MS with HPLC and interchangeable ESI/MALDI source

  • Bruker 11 7 tesla fticr ms

    Bruker 11.7 Tesla FT-ICR MS equipped with interchangeable ESI/MALDI source

  • Thermo ltq orbitrap velos ft ms

    Thermo LTQ-Orbitrap Velos FT-MS


HPLC

TMIC offers HPLC-based methods for identifying and quantifying carotenoids, polyphenols, chlorophylls, bile acids and thiols, as well as for lipidomics studies. Our lipidomics workflow includes reverse phase HPLC-ELSD, which separates lipid classes into 7 groups. FAMES and GC-MS analysis are then performed to identify and quantify the component fatty acid chains. Combinatorial Lipid Reconstruction software, developed by TMIC is used to reconstruct lipid molecules and calculate their concentrations. Our lipidomics assays are capable of identifying and quantifying 2000-3000 lipids per sample.

Specialized metabolite assays are also available, using fluorescence-based or UV-based HPLC assays. Specialized metabolite assays include polyphenols, phytochemicals, nucleotides/ nucleosides, vitamins, thiols, bile acids, exotic lipids and extracts.

Find out more about HPLC here.

TMIC houses 6 HPLC/UPLC systems that are dedicated to these analyses:

  • Gold beckman hplc

    Beckman System Gold HPLC with ELSD detector

  • Agilent hplc

    Agilent HPLC system with fluorescence detector

  • Waters Acquity UPLC system with UV detector (2 instruments)

  • Waters acquity uplc

    Waters NanoAcquity UPLC system

  • Dionex Ultimate 3000 Dual HPLC system with UV detector


LC-MS

TMIC has developed a number of direct injection and LC-MS methods for targeted and untargeted metabolomics. Tandem quadrupole and ion trap instruments are ideal for targeted and quantitative studies. Multiple reaction monitoring (MRM) is used on these instruments to selectively measure specific metabolites and their internal standard, providing absolute quantitation. Our TOF systems provide the high resolution and mass accuracy required for identification of unknowns in untargeted studies. Direct injection analysis has the advantage of speed, and we are currently able to identify and quantify up to 180 different metabolites in a single analysis from blood, urine, saliva, cerebrospinal fluid, tissue and cell extracts, including phospholipids, sphingolipids, lysophospholipids, acylcarnitines, amino acids, and biogenic amines.

TMIC also offers quantitative selective isotope labeling of metabolites (SILOM), which involves chemical modification techniques that are specific to amines, carboxylic acids and hydroxyl groups. This allows for efficient chemo-specific labeling of metabolites with 13C or 12C isotope tags. Spiking known quantities of 13C labelled compounds into biofluids labelled with 12C tags combined with high resolution MS analysis provides identification and quantification of hundreds of metabolites.

In untargeted studies, commercial software (SIMCA by Umetrics) and in-house developed software are used for data processing, including multivariate statistical analysis for identification of changes in metabolite abundance between groups of samples. Untargeted metabolomics studies also utilize UPLC separation followed by Q-TOF MS analysis, or FT-MS analysis, which can yield semi-quantitative data for up to >5000 features per analysis, in as little as 30 minutes.

Find out more about LC-MS here.

The following list of instrumentation includes tandem quadrupole, ion trap, and TOF instruments for targeted and untargeted studies:

  • Abi qtrap 4000 ms Abi qtrap 4000 ms 2

    ABI QTRAP 4000 MS with Turbo ESI spray probe (two instruments)

  • Bruker ion trap

    Bruker Ion Trap MS with Agilent HPLC system

  • Agilent 6490 ms

    Agilent 6490 Triple Quadrupole MS (2 instruments)

  • Bruker qtof ms

    Bruker Q-TOF MS with Agilent HPLC system

  • Waters wynapt hdms

    Waters Synapt High Defintion Q-TOF MS with ion mobility


MALDI-MS

TMIC is the first facility in Canada to offer MS tissue imaging of endogenous metabolites. This service is available on our high-resolution TOF and FTMS instruments equipped with a MALDI source. Identification and relative abundance information is used to reconstruct colorful ion maps, resulting in a two-dimensional spatial resolution of small molecules (and proteins) in tissue. We can achieve image pixel resolutions of 150 um using FTICR mass spectrometry, and 50 um on the Bruker Ultraflex III MALDI-TOF/TOF.

Find out more about MALDI-MS here.

MALDI-MS analysis is offered on the following instruments:

  • Bruker tof tof maldi

    Bruker TOF/TOF with MALDI-MS for imaging

  • 4800 maldi tof ms

    Applied Biosystems 4800 MALDI TOF/TOF mass spectrometer (3 instruments)

  • Bruker ultraflexiii maldi tof

    Bruker Ultraflex III MALDI-TOF/TOF mass spectrometer

  • Bruker 11 7 tesla fticr ms

    Bruker 11.7 Tesla FT-ICR MS equipped with interchangeable ESI/MALDI source


ICP-MS

TMIC also offers ICP-MS for quantitative metallomics. We routinely identify and quantify 20-40 metal ions in a wide variety of biological matrices.

A Perkin-Elmer Sciex Elan 6000 Quadrupole instrument is currently used for metal ion studies:

  • Perkin-Elmer Sciex Elan 6000 Quadrupole ICP-MS with a dual-stage detector


We request that any publications, posters, or oral presentations resulting from work performed by The Metabolomics Innovation Centre acknowledge TMIC. This allows the Centre to report information to our funding agencies, such as impact on Canadian researchers. Clients should also keep us apprised of any publications, posters, or oral presentations arising from work performed at TMIC.